CX-008634: Categorical Exclusion Determination

Vegetative Response to Metal Exposure in a Growing Media CX(s) Applied: B3.6 Date: 05/31/2012 Location(s): South Carolina Offices(s): Savannah River Operations Office

Office of NEPA Policy and Compliance

May 31, 2012
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Vegetative Response to Metal Exposure in a Growing Media
CX(s) Applied: B3.6
Date: 05/31/2012
Location(s): South Carolina
Offices(s): Savannah River Operations Office

The objective of this project is to determine vegetation's physiological response to metal exposure and other stressors in the growing media. 772-27B Lab 4 will contain two plant growth chambers with a refrigerator for sample storage. Activities will include: growing plants from seed in soil, agar and hydroponically and the monitoring of plant growth for signature responses. Pesticides/fungicides may be used to minimize damage to the plants by insects and fungi. After harvest, plants will be processed and analyzed in 772-27B Labs 3 and/or 17, and 772-25B Lab 2. Processing will consist of weighing and separation of plant by tissue (root, stem, leaf). Laboratory instruments and equipment including spectrometers, spectrophotometers, bioanalyzer, reverse transcription polymerase chain reaction equipment, and others will be used. Plants samples will then be sent to an off-site lab for genomic analysis.