In order to prepare for future transportation of spent nuclear fuel from shutdown nuclear power plant sites to eventual storage or disposal facilities, an evaluation of removing spent nuclear fuel from 14 shutdown sites was conducted. Shutdown sites are defined as those commercial nuclear power reactor sites where the nuclear power reactors have been shut down and the site has been decommissioned or is undergoing decommissioning. The evaluation was divided into four components: (1) characterizing the spent nuclear fuel inventory, (2) describing the on-site infrastructure, (3) evaluating the near-site transportation infrastructure and transportation experience, and (4) evaluating the actions necessary to prepare for and remove spent nuclear fuel from the shutdown sites. The shutdown sites evaluated are Maine Yankee, Yankee Rowe, Connecticut Yankee, Humboldt Bay, Big Rock Point, Rancho Seco, Trojan, La Crosse, Zion, Crystal River, Kewaunee, San Onofre, Vermont Yankee, and Fort Calhoun. These sites have no other operating nuclear power reactors at their sites and have also notified the U.S. Nuclear Regulatory Commission that their reactors have permanently ceased power operations and that nuclear fuel has been permanently removed from their reactor vessels. Shutdown reactors at sites having other operating reactors were not included in this evaluation.