Fermentative production of tricarboxylic acid cycle‐derived chemicals using cellulosic sugars Award Number: DE-EE0007565 CX(s) Applied: A9, B3.6 Bioenergy Technologies Office Date: 8/22/2016 Location(s): CA Office(s): Golden Field Office
Office of NEPA Policy and Compliance
September 7, 2016
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Fermentative production of tricarboxylic acid cycle‐derived chemicals using cellulosic sugars
Award Number: DE-EE0007565
CX(s) Applied: A9, B3.6
Bioenergy Technologies Office
Date: 8/22/2016
Location(s): CA
Office(s): Golden Field Office
The U.S. Department of Energy (DOE) is proposing to provide federal funding to Lygos, Inc. to develop a low pH, anaerobic fermentation process that utilizes genetically engineered yeast to convert cellulosic glucose derived from non-food biomass into aspartic acid.
Activities associated with the proposed project would include the design and development of genetically engineered strains of the yeast P. kudriavzevii, development and testing of a bench-scale (15L volume or less) fermentation process using the genetically modified yeast, and chemical purification of aspartic acid from the fermentation broth. All work would be performed at the Lygos facility in Berkeley, CA within their existing laboratories that are purpose-built for the type of activities being proposed. The facility has all applicable permits in place, and would not need additional permits, licenses, or authorizations for the proposed activities